Advanced Research on Animal Cell Technology

Advanced Research on Animal Cell Technology

作者: Alain O.A. Miller

ISBN: 9789401068819

出版时间: 2011-10-28

出版社: Springer

Alain O.A. Miller 0 0 0

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目录I.Cell-CellCommunicationandCellExtracellularMatrixInteractions.-ExtracellularMatrixCellDifferentiation.-StructureandFunctionoftheHeparanSulfateProteoglycans.-RolePlayedbyGapJunctionsinCell-CellCommunication.-Fibronectin-MediatedCellular(Inter)actions.-II.“Normal”Versus“Abnormal”Cells.-UseofCellLinesinBiotechnology.-WhyNotUsePrimaryCells.-III.LargeScaleCultivationofAnimalCells.-TechnologyofMassCultivation:AnOverview.-CellCultureinHollowFibreBioreactors.-PerfusionCulture.-GoodManufacturingPracticeintheProductionofBiologicalsbyCellCultures.-Biosensors.-IV.AnalysisoftheCell’sBehaviour.-EffectsofRetinoidsonGrowthDifferentiation.-PhosphoinositideMetabolismandControlofCellProliferation.-SomaticCellGeneticAnalysisofGrowthControl.-PromoterOrganizationofEukaryoticProtein-CodingGenes.-V.ModificationoftheCellGenome.-TailoringofanAnti-HumanPlacentalAlkalinePhosphataseImmunoglobulinUsingGeneticEngineering.-VacciniaVirusasanExpressionVector.-ExpressionandIntegrationofExogenousDNASeguencesTransfectedintoMammalianCells.-CooperationofOncogenesinMalignantTransformation.-VI.ApplicationsofAnimalCellCultures.-MolecularDissectionoftheCellCycle.-UseofMicrobeadsforCellTransplantation.-InfluenceofEpigeneticFactorsonEpidermalDifferentiationandMorphogenesis.-TheCharacterizationofFibroblastGrowthFactorandItsBiologicalEffectInVitroandInVivo.-CultureofHybridomas―ASurvey.-ApplicationsofChromatographyinDownstreamProcessing.-Generaldiscussion.I.Cell-CellCommunicationandCellExtracellularMatrixInteractions.-ExtracellularMatrixCellDifferentiation.-StructureandFunctionoftheHeparanSulfateProteoglycans.-RolePlayedbyGapJunctionsinCell-CellCommunication.-Fibronectin-MediatedCellular(Inter)actions.-II.“Normal”Versus“Abnormal”Cells.-UseofCellLinesinBiotechnology.-WhyNotUsePrimaryCells.-III.LargeScaleCultivationofAnimalCells.-TechnologyofMassCultivation:AnOverview.-CellCultureinHollowFibreBioreactors.-PerfusionCulture.-GoodManufacturingPracticeintheProductionofBiologicalsbyCellCultures.-Biosensors.-IV.AnalysisoftheCell’sBehaviour.-EffectsofRetinoidsonGrowthDifferentiation.-PhosphoinositideMetabolismandControlofCellProliferation.-SomaticCellGeneticAnalysisofGrowthControl.-PromoterOrganizationofEukaryoticProtein-CodingGenes.-V.ModificationoftheCellGenome.-TailoringofanAnti-HumanPlacentalAlkalinePhosphataseImmunoglobulinUsingGeneticEngineering.-VacciniaVirusasanExpressionVector.-ExpressionandIntegrationofExogenousDNASeguencesTransfectedintoMammalianCells.-CooperationofOncogenesinMalignantTransformation.-VI.ApplicationsofAnimalCellCultures.-MolecularDissectionoftheCellCycle.-UseofMicrobeadsforCellTransplantation.-InfluenceofEpigeneticFactorsonEpidermalDifferentiationandMorphogenesis.-TheCharacterizationofFibroblastGrowthFactorandItsBiologicalEffectInVitroandInVivo.-CultureofHybridomas―ASurvey.-ApplicationsofChromatographyinDownstreamProcessing.-Generaldiscussion.

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